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Yeast DNA Preparation
- (Gottschling Lab)
Rapid method for yeast genomic DNA isolation
- (Hahn Lab)
- Yeast Genomic DNA Preparation (high quality DNA) (Hahn Lab)
- Yeast quick plasmid DNA Prep (Hahn Lab)
- Yeast "smash-&-grab" DNA Preparation (Fangman/Brewer lab)
- (Fangman/Brewer lab)
- (Fangman/Brewer lab)
- (Breeden Lab)
- (Breeden Lab)
- (Donis Keller Lab)
Quick method for preparing yeast genomic DNA
- High Molecular Weight Yeast Liquid DNA Preparation (Donis Keller Lab)
For isolating intact, high molecular weight DNA from yeast cells for subcloning and rare cutting restriction enzyme analysis. One can expect a yield of 100-200 µg of DNA per prep.
- (GSCWU)
- Restriction Digests of High Molecular Weight Yeast DNA (Donis Keller Lab)
Restriction digests of YACs for mapping using rare cutting enzymes or more conventional restriction endonuclease digestion.
- (D. Amberg)
- DNA Isolation from Yeast (Corbett Lab)
- Yeast Miniprep (Corbett Lab)
Yeast RNA Preparation
- (Gottschling Lab)
Give protocol for RNA preparation using phenol/freezer method and glass bead method
- (Breeden Lab)
Yeast Protein Preparation
- (Gottschling Lab)
Rapid method for yeast genomic DNA isolation
- Whole Cell Extracts (Dohlman Lab)
This is a quick method for extracting total proteins from Yeast for SDS-PAGE and Western Blotting.
- (Dohlman Lab)
A quick method for extracting total proteins from Yeast for SDS-PAGE and Western Blotting (same protocol as above)
- (Haha Lab)
- Yeast Protein Preparation for SDS PAGE and Western (Haha Lab)
Yeast whole cell protein extraction for western blot using glass bead method
· (Dohlman Lab)
This is an excellent protocol for localization of membrane associated proteins in yeast.
· Protein Extraction from S. cerevisiae (Howell Lab)
Glass bead method
· Extracts for Yeast Westerns (Herskowitz Lab)
· Yeast Protein Isolation (Amberg Lab)
Yaffe-Schatz method
· Yeast Nuclear Extract Prep (small scale) (Hahn Lab)
· Yeast Whole Cell Extract (small scale) (Hahn Lab)
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