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· Analysis of Protein by Immunoprecipitatioin (P.J. Hansen Lab)
This protocol describes two methods (protein A and G) in great details as well as the principal, application and trouble shooting of immunoprecipitation.
· (LTI)
Integrin receptors on cell surfaces are labeled with biotin; the receptors are then extracted from the membranes by detergent treatment and saved for immunoprecipitation. Integrin-specific antibodies are bound to Sepharose beads coated with anti-IgG and added to the biotinylated cell extract. Bound receptors are run on an SDS-PAGE, transferred to nitrocellulose, and detected by the addition of streptavidin-enzyme conjugate followed by substrate.
· (Transduction Laboratories)
Provides detailed protocol on immunoprecipitation assay.
· (Transduction Laboratories)
Provides detailed protocol on immunoprecipitation assay.
· (Transduction Laboratories)
Provides detailed protocol on immunoprecipitation assay.
· Immunoprecipitation from Eukaryotic Cell Extracts (PMCI)
· (Sefton Lab)
· (Daniel T. O'Connor)
· Immunoprecipitation (Hancock Lab)
· Glycolipid Binding assay (Paller’s Lab, Contributed by Pingsunjim)
This protocol can be used for the detection of glycolipids binding to immunoprecipited protein.
· Antibody precipitation with Protein A or G (Contributed by James Turmo )
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