· (Aberdeen's Lab)
The method used to locate the proteins following 2D-PAGE depends on the nature of the original sample.
· Protein detection(Silver staining) (ExPASy)
The application of the 2-D PAGE technology to separate, analyze and characterize proteins contained in biological samples would not have been possible without the development of complementary detection methods. Perhaps today one of the most popular non-radioactive protein detection is the silver staining which is 100-fold more sensitive than Coomassie Brilliant Blue staining
· Indirect Immunofluorescencc (Brent Graveley)
· Coomasie Blue Staining of Protein Gel (William H. Heidcamp)
Coomasie Brilliant Blue R 250 is the most commonly used staining procedure for the detection of proteins. It is the method of choice if SDS is used in the electrophoresis of proteins, and is sensitive for a range of 0.5 to 20 micrograms of protein.
· CDP-Star Western Blot Detection (Gimila Lab)
· Silver Staining of Protein Gels (William H. Heidcamp)
· (Waters Lab)
· Silver Staining of Proteins in Gel (Contributed by Sharad Purohit)
· (Contributed by Sharad Purohit)
· (Hahn Lab)
· Phosphoamino Acid Analysis of non-TCA Precipitable Proteins (Woodgett Lab)
· SDS PAGE Staining Protocol (LTI)
