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· (Crawford Lab)
mRNA can be converted into DNA (copy DNA, cDNA) by annealing oligo-dT to the 3' poly-A tail that occurs on all eukaryotic mRNA. After the dTs bind to the As, we will use the enzyme "Reverse Transcriptase" to read the RNA into DNA
· (LTI)
Protocol for Semi-solid Amplification of Plasmid cDNA Libraries
· (Promega)
Provides detailed procedures for construction of cDNA library, including first strand and double-stranded cDNA synthesis from mRNA, and subsequent ligation into a suitable vector.
· (Fermentas)
· cDNA Library Construction (Michael Blaber's Lab)
Very useful guide and protocol for cDNA library construction
· (HMG)
Detailed protocol for cDNA cloning
· (Gerard Lazo)
cDNA library construction and differential screening
- cDNA Synthesis (Cungui Mao)
- nsitu.html">Screening cDNA Clones by RNA In Situ Hybridization (Berkeley Drosophila Genome Project)
The RNA in situ procedure described below is based on the protocol developed by Tautz and Pfeifle, but adapted to allow the screening of 96 RNA probes on whole-mount Drosophila embryos at the same time.
· 32P Labeled Single-Stranded cDNA Synthesis (Jim Brown Lab)
32P labeled single-stranded cDNA (sscDNA) can be used in a usr/localiety of techniques; as a hybridization probe, for renaturation studies, or for eventual cloning.
- cDNA/AFLP (CWB Bachem)
The protocol which follows gives a step-by-step description of cDNA-AFLP, a method for visualisation of differential gene expression
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