| 146种培养基配方（细菌培养基与植物培养基） | | 点击： 作者： 来源： 时间： 2007-05-09 本站论坛 |
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18. Corn Meal Medium (玉米粉培养基)
Mix corn meal with water [20:100(W/V)] well . Distribute the mixture into 100ml bottles , each bottle contains about 30ml of the mixture . Autoclave at 121℃ for 30minutes . (20%玉米粉装瓶，15磅灭菌30分钟。)
19. Filter Paper Medium （滤纸培养基）
（NH4）2SO4 1g KH2PO4 1g
MgSO4.7H2O 0.7g NaCl 0.5g
Distilled water （蒸馏水） 1000ml A strip of filter paper （滤纸条） 6ⅹ1cm （厘米）
20. Soybean Cake Meal Agar (黄豆饼粉琼脂)
10% infusion of soybean cake meal (10%黄豆饼粉浸汁) 1000ml
Glucose (葡萄糖) 10g NaCl 2.5g
21. Sawdust， Wheat-bran Medium （木屑、麸皮培养基）
Sawdust of broad leaf tree 75g Wheat bran 25g
Preparation：Mix these two ingredients well . Add appropriate water and blend (the amount of water added may be becided by kneading with fingers ). Fill the mixture into broad mouth of bottles . Stick a hole on the filling, put the cotton pulg in bottle mouth .
壳斗科植物或阔叶树的木屑 75克 麸皮 25克
制法：加水适量充分混匀，水量以手捏时指间有水珠为宜，装瓶并压紧，在中心处扎一小洞，堵上棉塞，20磅灭菌1小时。
22. Pine Block or Pine Sowdust Medium （松木条或松木屑培养基）
1. Cut 1021 cm pine biocks and immerse them in 1-2% sucrose solution . Alow the biocks fully absorbing suger solution .Fill the biocks into broad month of bottle . Park the bottle with paper . Autoclave at 20 lbs for 1 hr . (将10ⅹ2ⅹ1厘米的松木条浸泡在1-2%的蔗糖水中。使木条吸足糖水，装瓶不宜过紧，用纸包好，20磅灭菌1小时。)
2. Pine Sawdust Medium
Pine Sawdust (松木屑) 75% Rice bran (米糠) 23-24%
Socrose (蔗糖) 1-2% Water (appropriate)水 （适量）
Autoclave at 20 lbs for 1 hr.(20磅灭菌1小时）
23. Rice Straw. Dung Medium (稻草、马粪培养基)
Dried horse dung (or cow dung) （干马粪或牛粪） 65%
Died straw （干稻草） 35% Urea （尿素） 0.5%
Calcium superhosphate （过磷酸钙）0.7% Gypsum powder（石膏粉） 1%
Ammonium sulphate （硫酸铵） 0.3%
[Note]: Add appropriate water, pile them up and cause them fermentation. （水适量进行堆积发酵）
24. Straw Rice Bran Medium （稻草、米糠培养基）
Dried straw （干稻草） 75-80% Rice bran （米糠） 20-25%
[Note]：Select no mouldy straw and cut them about 3 cm in length. Immerse them in fresh water for 12 hr. Allow the straw fully (the amount of water added may be decided by kneading with fingers. It is right when a drop of water appeared between fingers). ( [注]：选无霉稻草，切成长约3厘米的小段，用清水浸泡12小时，使充分吸水，然后倾去水，加米糠和水，水量以用手捏时指间有水珠为宜。)
25. Rice Medium (米饭培养基)
Fill 10g of rice into a 50ml of Erlenmeyer flask. Add 20-30ml water. Autoclave at 121℃ for 30 minutes (or fill 2g of rice into a 15ⅹ150mm tube). Add 6 ml of water. Autoclave at 121℃ for 30 minutes. (取大米10克，入50毫升椎形瓶中，水20-30毫升于15磅灭菌30分钟即成，或取大米2克，装入15ⅹ150毫米试管中，加水6毫升，于15磅灭菌30分钟。)
26. Filter Paper Medium (滤纸条培养基)
(NH4)2SO4 1g KH2PO4 1g
MgSO4.7H2O 0.5g Yeast Extract (酵母膏) 0.1g
Distilled Water (蒸馏水) 1000ml
Strip of filter paper 1ⅹ7 cm (滤纸条 1ⅹ7 厘米)
[Note]：Siddolve the above ingredients. Distribute the liqid medium into tubes (each tube contains 1ml of the broth). Autoclave at 121℃ for 30 minutes. Then put one or two sterilized strips of filter paper into a tube aseptically. (将上述溶液分配成溶液后分装入试管，每管1毫升，15磅灭菌30分钟。再将灭菌的滤纸条（1条或2条）投入试管中即好使用。)
27. Yeast Extract-Medium Extract Agar I (酵母膏、麦芽膏琼脂)
Yeast extract (酵母膏) 10g Mall extract (麦芽膏) 10g
Glucose (葡萄糖) 4g Distilled water (蒸馏水) 1000ml
Agar (琼脂) 20g pH 7.0
适用范围：红霉素链霉菌
28. Yeast Extract Peptone （酵母膏、蛋白胨琼脂）
Yeast extract （酵母膏） 1g Multi-peptone（多蛋白胨） 2g
Beef extract （牛肉膏） 1g Glucose （葡萄糖） 10g
Agar （琼脂） 20g Distilled water （蒸馏水） 1L
pH 7.0
29. Glucose Yeast Extract Starch Agar （葡萄糖、酵母、淀粉琼脂）
Glucose （葡萄糖） 10g Yeast extract （酵母膏） 10g
Starch （淀粉） 10g NaCl 5g
CaCO3 3g Agar （琼脂） 20g
Distilled water （蒸馏水） 1L pH 7.0
30. Wheat Bran Medium （麸皮培养基）
Wheat bran （麸皮） 36g （NH4）2HPO4 10g
K2HPO4 0.2g MgSO4·7H2O 0.1g
Agar （琼脂） 20g Distilled water （蒸馏水） 1L
pH 7.0
31. Yeast Sporulation Medium (酵母菌产生子囊孢子培养基)
(1) Kleyn Medium
KH2PO4 0.012% K2HPO4 0.02%
Sodium acetate (醋酸钠) 0.5% Glucose (葡萄糖) 0.062%
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