1. Bring sections to water
2. Stain in Celestine blue, 3 mins
3. Wash in water
4. Stain in Carazzi Haemalum 3 minutes 5. Wash in water
6. Blue in Scotts tap water
7. Wash in water
8. Rinse in 95% alcohol
9. Stain in Picro-orange 2 mins
10. Wash in water until only red blood cells are yellow
11. Stain in acid fuchsin 5 mins
12. Wash in water
13. Stain in equal parts Picro-orange and 80% alcohol
14. Differentiate in phosphomolybdic acid for 8 mins
15. Wash in water
16. Stain in analine blue, 8 mins
17. Wash in water
18. Dehydrate, clear . Mount sections in DPX
RESULTS:
--Fibrin - Bright red
--Muscle - Red
--Collagen - Blue
--Red blood cells - Yellow/Orange
SOLUTIONS:
1. 0.2% orange G in saturated alcoholic Picric Acid
2. 1% Acid fuchsin in 3% Trichloroacetic Acid
3. 1% Phosphomolybdic Acid
4. 2% Analine blue W5 in 2% Acetic Acid (soluble Blue)
NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.
