| Department of Psychology, SUNY at Albany
http://www.albany.edu/psy/obssr3/protocols/husbandr.html
Five periods may differ in the requirements to some extent. Features mentioned only for the first period are common to all periods.
After arrival from supplier but before mating; housed with same sex
Mated pairs before and after delivery of litter
Weaned offspring before the start of behavioral testing
Preparation for the formal behavioral testing - may be specific to this study
During behavioral testing
After arrival
Food Purina 5001 (4.5% fat)
Bedding Bed-O-Cob 1/4"
Nesting material none
Cage top Stainless steel bars (plus passive filter top in Portland)
Animal handling Gloved hand
Animal marking nil
Animal weighing nil
Mice/cage 4 or 5 maximum
Lights on/off 0600/1800
Mated pairs (items the same as after arrival are not repeated)
Food Purina 5020 (9% fat)
Nesting material 1 Nestlet
Marking none
Weighing at mating
Mice/cage 1 female, 1 male
Male present with litter? Yes; postpartum pregnancy allowed
Inspection of new litter Count live and dead pups; remove dead, put live in nest
Culling of newborns Remove only the obvious runts for euthanasia
Cage changing with litter Do not disturb cage until litter is 4 days old; then clean cage, including fresh nestlet
Weaned offspring
Age at weaning Aim for 21 days (may be ± 1 day when weekend involved)
Weighing Record body weight to nearest 0.5g; find means for each sex
Food Purina 5001
Mice/cage Same sex littermates, 4 or 5 maximum
Marking nil
About two weeks before the start of behavioral testing
Mice/cage Cull to 2 by random lottery (keep some spares in reserve)
Cage changing day Friday
During behavioral testing
Marking Sharpie pen (black or red) on tail; start Monday AM
Weighing On Friday before the coke test
Handling Hand with plastic surgical glove
Cage changing day Friday (AFTER coke test trial for each pair)
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