| Crutcher Lab, University of Cincinnati
http://crutcherlab.med.uc.edu/costain.htm
1.) Prepare 0.01% thioflavin S (T1892 , Sigma) solution in F12 [0.01g / 100 ml F12 ]
2.) Filter the staining solution with a 0.22 µm Millipore filter.
3.) Incubate the staining solution and F12 wash for approximately 30 minutes to adjust solution temperature to 37°C
4.) Stain with thioflavin S for 15 minutes
5.) Wash with F12 once
6.) Stain with carboxyfluorescein 1:200 (C1354 Molecular Probes,1mM w/DMSO) for 30 minutes
7.) Wash with F12 twice
8.) View immediately under the fluorescent microscope using FITC filter.
9.) Fix with 2% formalin for 20 to 30 minutes
10.) Coverslip with water-soluble mounting media
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