MethPrimer - Design Primers for Methylation PCRs

• MethPrimer is a program for designing bisulfite-conversion-based Methylation PCR Primer. Currently, it can design primers for two types of methylation mapping PCRs: 1) Methylation-Specific PCR (MSP) and 2) Bisulfite-Sequencing PCR (BSP) or Bisulfite-Restriction PCR.
• Input Sequence: DNA sequences in any format. No editing is required before input.
• Output: MethPrimer returns results in both text and graphic view.

How to cite MethPrimer: Li LC, Dahiya R. MethPrimer: designing primers for methylation PCRs. Bioinformatics. 2002 Nov;18(11):1427-31.

Discussion forum for methylation related methods discussion (moderated) Join the CpG method forum to ask questions and share your knowledge on methylation related methods, such as bisulfite modification, primer design, MSP, bisulfite sequencing PCR.

Acknowledgement: MethPrimer is based on on the well-known primer design program Primer3 (Steve Rozen, Helen J. Skaletsky (1998) Primer3. Code available at http://www-genome.wi.mit.edu/genome_software/other/primer3.html. Copyright (c) 1996,1997,1998 Whitehead Institute for Biomedical Research. All rights reserved. ).

MethPrimer was developed by Dr. Long-Cheng Li at Dr. Dahiya's Urology Research Center, Veterans Affairs Medical Center and University of California, San Francisco.

Send bug reports and feature requests to longli@itsa.ucsf.edu
Page last updated: November 13, 2002

http://www.ucsf.edu/urogene/methprimer/index1.html